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MUC1/CA15-3 C-terminal Recombinant antibody

MUC1/CA15-3 C-terminal Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240190G10

Cat no : 83311-4-RR

Synonyms

CA15-3, CA153, MUC1, CA15 3, CA 15-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MUC1/CA15-3 C-terminal Recombinant antibody (83311-4-RR)

    WB analysis using 83311-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83311-4-RR (MUC1/CA15-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83311-4-RR

    Affinity and Kinetic Characterization of 83311-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83311-4-RR against Human CA15-3/MUC1 were performed. The affinity constant is 63.7 pM.

Tested Applications

Positive WB detected inBxPC-3 cells, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83311-4-RR is MUC1/CA15-3 C-terminal Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MUC1/CA15-3 C-terminal fusion protein Ag20366
Full Name mucin 1, cell surface associated
Calculated Molecular Weight 1264 aa, 123 kDa
Observed Molecular Weight 23-30 kDa
GenBank Accession NumberBC120975
Gene Symbol MUC1/CA15-3
Gene ID (NCBI) 4582
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.

Protocols

Product Specific Protocols
WB protocol for MUC1/CA15-3 C-terminal antibody 83311-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols