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PGT Recombinant antibody

PGT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230139F5

Cat no : 83302-1-RR

Synonyms

SLCO2A1, PHOAR2, OATP2A1, 230139F5

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using PGT Recombinant antibody (83302-1-RR)

    WB analysis of NIH/3T3 using 83302-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using PGT Recombinant antibody (83302-1-RR)

    WB analysis of mouse heart using 83302-1-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83302-1-RR (PGT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using PGT Recombinant antibody (83302-1-RR)

    FC experiment of HeLa using 83302-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PGT Recombinant antibody (83302-1-RR, Clone:230139F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83302-1-RR

    Affinity and Kinetic Characterization of 83302-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83302-1-RR against Human PGT were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse heart tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83302-1-RR is PGT Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PGT fusion protein Ag5594
Full Name solute carrier organic anion transporter family, member 2A1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC041140
Gene Symbol PGT
Gene ID (NCBI) 6578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).

Protocols

Product Specific Protocols
WB protocol for PGT antibody 83302-1-RRDownload protocol
FC protocol for PGT antibody 83302-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols