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GINS1 Recombinant antibody

GINS1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240234A2

Cat no : 83293-1-RR

Synonyms

PSF1, KIAA0186, GINS complex subunit 1, 240234A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GINS1 Recombinant antibody (83293-1-RR)

    WB analysis using 83293-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83293-1-RR (GINS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83293-1-RR

    Affinity and Kinetic Characterization of 83293-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83293-1-RR against Human GINS1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83293-1-RR is GINS1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GINS1 fusion protein Ag8895
Full Name GINS complex subunit 1 (Psf1 homolog)
Calculated Molecular Weight 196 aa, 23 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC012542
Gene Symbol GINS1
Gene ID (NCBI) 9837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GINS1, also known as Partner of SLD Five 1 (PSF1) is a member of the GINS (Go-Ichi-Nii-San) complex, which plays a vital role in the initiation and elongation of DNA replication (PMID: 34414190). GINS1 was initially found to be predominantly expressed in highly proliferating cells instead of mature cells. Later on, it was found that GINS was involved in the tumorigenesis of various cancers, including breast cancer, colorectal cancer, and hepatocellular carcinoma (PMID: 36065190).

Protocols

Product Specific Protocols
WB protocol for GINS1 antibody 83293-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols