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GAPDHS Recombinant antibody

GAPDHS Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240277F1

Cat no : 83290-3-RR

Synonyms

GAPD2, GAPDH 2, GAPDH2, GAPDHS, GAPDS, HSD 35

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAPDHS Recombinant antibody (83290-3-RR)

    WB analysis using 83290-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83290-3-RR (GAPDHS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83290-3-RR

    Affinity and Kinetic Characterization of 83290-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83290-3-RR against Human GAPDHS were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83290-3-RR is GAPDHS Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GAPDHS fusion protein Ag4988
Full Name glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Calculated Molecular Weight 45 kDa
Observed Molecular Weight60-65 kDa
GenBank Accession NumberBC036373
Gene Symbol GAPDHS
Gene ID (NCBI) 26330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAPDHS(Glyceraldehyde-3-phosphate dehydrogenase, testis-specific) is also named as GAPD2, GAPDH2, GAPDS and belongs to the glyceraldehyde-3-phosphate dehydrogenase family. It may play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. GAPDHS is a promising contraceptive target because it is specific to male germ cells, essential for sperm motility and male fertility, and well suited to pharmacological inhibition(PMID:20828617).

Protocols

Product Specific Protocols
WB protocol for GAPDHS antibody 83290-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols