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  • Flow cytometry (FC) experiment of U251 using KERA Recombinant antibody (83272-1-RR)

    FC experiment of U251 using 83272-1-RR

    1x10^6 U251 were intracellularly stained with 0.2 ug KERA Recombinant Antibody (83272-1-RR, Clone:230399B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83272-1-RR

    Affinity and Kinetic Characterization of 83272-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83272-1-RR against Human KERA were performed. The affinity constant is below 1 pM.

KERA Recombinant antibody

KERA {ptg:Brand} Recombinant Antibody for FC (Intra), ELISA
Cat No. 83272-1-RR
Clone No.230399B11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

Keratocan, SLRR2B, KTN, CNA2, 230399B11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC (Intra) detected inU251

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83272-1-RR targets KERA in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KERA fusion protein Ag34171 Predict reactive species
Full Name keratocan
Calculated Molecular Weight 352 aa, 41 kDa
GenBank Accession NumberBC032667
Gene Symbol KERA
Gene ID (NCBI) 11081
RRIDAB_3670944
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60938
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratocan and Lumican are the predominant class II SLRPs(Small leucine-rich proteoglycans) in the corneal stroma. While not totally corneal-specific together these SLRPs are excellent biomarkers for the tissue. Mutant mice deficient for keratocan expression have transparent but thin corneal stromas with a mild increase in collagen fibril diameter with no further abnormalities.In humans, mutations in the keratocan gene are associated with a flattened cornea in a rare condition known as cornea plana. The relative specificity of keratocan to corneal keratocytes has been useful for tissue-specific targeting for genetic manipulation of the corneal stroma.(PMID: 32663498)

Protocols

Product Specific Protocols
FC protocol for KERA antibody 83272-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols