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BUB3 Recombinant antibody, PBS Only (Capture/Detector)

BUB3 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240087C3

Cat no : 83266-1-PBS

Synonyms

hBUB3, BUB3L, BUB 3, 240087C3

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00297-3

    Sandwich ELISA standard curve of MP00297-3

    Sandwich ELISA standard curve of MP00297-3, Human BUB3 Recombinant Matched Antibody Pair - PBS only. 83266-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25608. 83266-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP00297-3

    Sample test of MP00297-3

    The mean BUB3 concentration was determined to be 30.78 ng/mL in Jurkat cell extract based on a 1.00 mg/mL extract load and 27.28 ng/mL in HEK-293T cell extract based on a 1.00 mg/mL extract load.

  • Cytometric bead array standard curve of MP00297-2

    Cytometric bead array standard curve of MP00297-2

    Cytometric bead array standard curve of MP00297-2, BUB3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83266-1-PBS. Detection antibody: 83266-3-PBS. Standard: Ag25608. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00297-3

    Cytometric bead array standard curve of MP00297-3

    Cytometric bead array standard curve of MP00297-3, BUB3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83266-2-PBS. Detection antibody: 83266-1-PBS. Standard: Ag25608. Range: 1.25-20 ng/mL.

Product Information

83266-1-PBS targets BUB3 as part of a matched antibody pair:

MP00297-2: 83266-1-PBS capture and 83266-3-PBS detection (validated in Cytometric bead array)

MP00297-3: 83266-2-PBS capture and 83266-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BUB3 fusion protein Ag25608
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC005138
Gene Symbol BUB3
Gene ID (NCBI) 9184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.