• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

nectin 4 Recombinant antibody, PBS Only (Detector)

nectin 4 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240009B8

Cat no : 83223-7-PBS

Synonyms

Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, LNIR

Validation Data Gallery

Filter:
  • Sandwich ELISA standard curve of MP00147-4

    Sandwich ELISA standard curve of MP00147-4

    Sandwich ELISA standard curve of MP00147-4, Human Nectin-4/PVRL4 Recombinant Matched Antibody Pair - PBS only. 83223-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0415. 83223-7-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/ml.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    Urine of eight individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 3,920.62 pg/mL with a range of 249.12 - 9,633.39 pg/mL.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. 1 x 10^6 cells/mL were lysated by RIPA buffer, and the supernate was assayed and measured 3.2 ng/mL.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    The mean Nectin-4/PVRL4 concentration was determined to be 102.1 ng/mL in MDA-MB-468 cell extract based on a 3.1 mg/mL extract load and 107.4 ng/mL in MCF-7 cell extract based on a 4.2 mg/mL extract load.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    Serum of sixteen individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 750.17 pg/mL with a range of 392.48 - 1,810.23 pg/mL.

Product Information

83223-7-PBS targets nectin 4 as part of a matched antibody pair:

MP00147-4: 83223-1-PBS capture and 83223-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name poliovirus receptor-related 4
Calculated Molecular Weight 510 aa, 55 kDa
GenBank Accession NumberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.