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Nectin-4/PVRL4 Recombinant antibody

Nectin-4/PVRL4 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

240009E2

Cat no : 83223-6-RR

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nectin-4/PVRL4 Recombinant antibody (83223-6-RR)

    WB analysis using 83223-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83223-6-RR (Nectin-4/PVRL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using Nectin-4/PVRL4 Recombinant antibody (83223-6-RR)

    FC experiment of MCF-7 using 83223-6-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Nectin-4/PVRL4 Recombinant antibody (83223-6-RR, Clone: 240009E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman placenta tissue, A2780 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83223-6-RR is Nectin-4/PVRL4 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name poliovirus receptor-related 4
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC010423
Gene Symbol PVRL4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

Protocols

Product Specific Protocols
WB protocol for Nectin-4/PVRL4 antibody 83223-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols