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nectin 4 Recombinant antibody, PBS Only (Detector)

nectin 4 Uni-rAbTM Recombinant Antibody for Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240009F8

Cat no : 83223-3-PBS

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4

Validation Data Gallery

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  • Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2, nectin 4 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83223-1-PBS. Detection antibody: 83223-3-PBS. Standard: Eg0415. Range: 0.625-10 ng/mL.

Product Information

83223-3-PBS targets nectin 4 as part of a matched antibody pair:

MP00147-2: 83223-1-PBS capture and 83223-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=5.81 x 10-10M
KOff=1.47 x 10-4M
KOn=2.53 x 105M
Immunogen Recombinant protein
Full Name poliovirus receptor-related 4
Calculated Molecular Weight 510 aa, 55 kDa
GenBank Accession NumberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).