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KIM-1/HAVCR1 Recombinant antibody

KIM-1/HAVCR1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Rat, Mouse, Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230528C5

Cat no : 83221-2-RR

Synonyms

HAVCR1, KIM-1, TIM-1, Kim1, KIM 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIM-1/HAVCR1 Recombinant antibody (83221-2-RR)

    WB analysis using 83221-2-RR

    Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 83221-2-RR (HAVCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KIM-1/HAVCR1 Recombinant antibody (83221-2-RR)

    WB analysis using 83221-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83221-2-RR (HAVCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83221-2-RR

    Affinity and Kinetic Characterization of 83221-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83221-2-RR against Rat HAVCR1 were performed. The affinity constant is 0.477 nM.

Tested Applications

Positive WB detected inmouse kidney tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83221-2-RR targets KIM-1/HAVCR1 in WB, ELISA applications and shows reactivity with Rat, Mouse, Human samples.

Tested Reactivity Rat, Mouse, Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name hepatitis A virus cellular receptor 1
Calculated Molecular Weight34 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberNM_173149.2
Gene Symbol Havcr1
Gene ID (NCBI) 286934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1), CD365, or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV) (PMID: 9658108; 8861957). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance (PMID: 16284246; 15793575). KIM-1 may be involved in the regulation of asthma and allergic diseases (PMID: 14534576). It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury (PMID: 14600030).

Protocols

Product Specific Protocols
WB protocol for KIM-1/HAVCR1 antibody 83221-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols