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  • Western Blot (WB) analysis of various lysates using JUND Recombinant antibody (83134-6-RR)

    WB analysis using 83134-6-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 83134-6-RR (JUND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JUND Recombinant antibody (83134-6-RR)

    IF Staining of HeLa using 83134-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUND antibody (83134-6-RR, Clone: 230457A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using JUND Recombinant antibody (83134-6-RR)

    FC experiment of A431 using 83134-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug JUND Recombinant antibody (83134-6-RR, Clone:230457A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

JUND Recombinant antibody

JUND Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83134-6-RR
Clone No.230457A4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

AP 1, jun D proto oncogene, JUND, Transcription factor jun D

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83134-6-RR targets JUND in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name jun D proto-oncogene
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35-45 kDa
GenBank Accession NumberNM_005354
Gene Symbol JUND
Gene ID (NCBI) 3727
RRIDAB_3670836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17535
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for JUND antibody 83134-6-RRDownload protocol
IF protocol for JUND antibody 83134-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols