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  • Featured Product
  • KD/KO Validated

MTCO2 Recombinant antibody

MTCO2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230448C9

Cat no : 83132-7-RR

Synonyms

COX2, MT CO2, COXII, COX 2, COII

Validation Data Gallery

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  • Western Blot (WB) analysis of 143B.TK.P0 cells using MTCO2 Recombinant antibody (83132-7-RR)

    WB analysis of 143B.TK.P0 using 83132-7-RR

    WB result of MTCO2 antibody (MTCO2; 1:3000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

  • Western Blot (WB) analysis of various lysates using MTCO2 Recombinant antibody (83132-7-RR)

    WB analysis using 83132-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MTCO2 Recombinant antibody (83132-7-RR)

    IP experiment of HeLa using 83132-7-RR

    IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using MTCO2 Recombinant antibody (83132-7-RR)

    IHC staining of human ovarian cancer using 83132-7-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MTCO2 Recombinant antibody (83132-7-RR)

    IHC staining of human stomach cancer using 83132-7-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTCO2 Recombinant antibody (83132-7-RR)

    IF Staining of HepG2 using 83132-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using MTCO2 Recombinant antibody (83132-7-RR)

    FC experiment of HeLa using 83132-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHepG2 cells, 143B.TK.P0 cells, 143B cells, HeLa cells, U-251 cells, A549 cells, MDA-MB-231 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovarian cancer, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83132-7-RR targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name cytochrome c oxidase II
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberYP_003024029
Gene Symbol MTCO2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Protocols

Product Specific Protocols
WB protocol for MTCO2 antibody 83132-7-RRDownload protocol
IHC protocol for MTCO2 antibody 83132-7-RRDownload protocol
IF protocol for MTCO2 antibody 83132-7-RRDownload protocol
IP protocol for MTCO2 antibody 83132-7-RRDownload protocol
FC protocol for MTCO2 antibody 83132-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols