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SHBG Recombinant antibody, PBS Only (Detector)

SHBG Uni-rAbTM Recombinant Antibody for Cytometric bead array, Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230356F10

Cat no : 83096-1-PBS

Synonyms

Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin, SBP

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using SHBG Recombinant antibody (83096-1-RR)

    WB analysis of human placenta using 83096-1-RR (same clone as 83096-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83096-1-RR (SHBG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83096-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SHBG Recombinant antibody (83096-1-RR)

    FC experiment of HeLa using 83096-1-RR (same clone as 83096-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SHBG Recombinant antibody (83096-1-RR, Clone:230356F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83096-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80006-1

    Cytometric bead array standard curve of MP80006-1

    Cytometric bead array standard curve of MP80006-1, SHBG Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 68835-1-PBS. Detection antibody: 83096-1-PBS. Standard: Eg0369. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83096-1-RR

    Affinity and Kinetic Characterization of 83096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83096-1-RR against Human SHBG were performed. The affinity constant is 0.38 nM.

Product Information

83096-1-PBS targets SHBG as part of a matched antibody pair:

MP80006-1: 68835-1-PBS capture and 83096-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name sex hormone-binding globulin
Calculated Molecular Weight 402 aa, 44 kDa
GenBank Accession NumberBC101785
Gene Symbol SHBG
Gene ID (NCBI) 6462
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.