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EPOR Recombinant antibody

EPOR Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230117G3

Cat no : 83056-4-RR

Synonyms

EPO R, EPOR, erythropoietin receptor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of TF-1 cells using EPOR Recombinant antibody (83056-4-RR)

    IF Staining of TF-1 using 83056-4-RR

    Immunofluorescent analysis of (4% PFA) fixed TF-1 cells using EPOR antibody (83056-4-RR, Clone: 230117G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IF/ICC detected inTF-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83056-4-RR targets EPOR in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPOR fusion protein Ag33956
Full Name erythropoietin receptor
Calculated Molecular Weight55 kDa
GenBank Accession NumberNM_000121.4
Gene Symbol EPOR
Gene ID (NCBI) 2057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Erythropoietin (EPO) receptor (EPOR) is a glycoprotein that belongs to the type I superfamily of single-transmembrane cytokine receptors. It consists of an extracellular domain that binds to the EPO ligand, a transmembrane domain and an intracellular domain. The interaction of EPO and EPOR triggers the activation of several signaling pathways that induce erythropoiesis, including JAK2/STAT5, PI3K/AKT, and MAPK (PMID: 34281163). EPOR is present on erythroid progenitor cells and has also been detected on a wide variety of non-hematopoietic cells (PMID: 36233351).

Protocols

Product Specific Protocols
IF protocol for EPOR antibody 83056-4-RRDownload protocol
FC protocol for EPOR antibody 83056-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols