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VEGFR2/KDR Recombinant antibody

VEGFR2/KDR Uni-rAbTM Recombinant Antibody for WB, IHC, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230238D7

Cat no : 83049-4-RR

Synonyms

KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using VEGF Receptor 2 Recombinant antibody (83049-4-RR)

    WB analysis of human placenta using 83049-4-RR

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (KDR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGF Receptor 2 Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGF Receptor 2 Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HUVEC cells using VEGFR2/KDR Recombinant antibody (83049-4-RR)

    FC experiment of HUVEC using 83049-4-RR

    1x10^6 HUVEC cells were surface stained with 0.25 ug VEGF Receptor 2 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 83049-4-RR

    Affinity and Kinetic Characterization of 83049-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

83049-4-RR targets VEGFR2/KDR in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Calculated Molecular Weight152kd
Observed Molecular Weight150 kDa, 230 kDa
GenBank Accession NumberNM_002253
Gene Symbol KDR
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Protocols

Product Specific Protocols
WB protocol for VEGFR2/KDR antibody 83049-4-RRDownload protocol
IHC protocol for VEGFR2/KDR antibody 83049-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biol Toxicol

HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells

Authors - Hanting Wang
View Article