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  • Featured Product
  • KD/KO Validated

ABCD1 Recombinant antibody

ABCD1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230226E12

Cat no : 83038-4-RR

Synonyms

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ABCD1 Recombinant antibody (83038-4-RR)

    WB analysis of HeLa using 83038-4-RR

    WB result of ABCD1 antibody (83038-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCD1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using ABCD1 Recombinant antibody (83038-4-RR)

    WB analysis using 83038-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83038-4-RR (ABCD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ABCD1 Recombinant antibody (83038-4-RR)

    IF Staining of HeLa using 83038-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ABCD1 antibody (83038-4-RR, Clone: 230226E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using ABCD1 Recombinant antibody (83038-4-RR)

    FC experiment of HeLa using 83038-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ABCD1 Recombinant antibody (83038-4-RR, Clone:230226E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83038-4-RR

    Affinity and Kinetic Characterization of 83038-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83038-4-RR against Human ABCD1 were performed. The affinity constant is 2.26 nM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, JAR cells, mouse liver tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83038-4-RR targets ABCD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ABCD1 fusion protein Ag0453
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Calculated Molecular Weight 745 aa, 83 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC025358
Gene Symbol ABCD1
Gene ID (NCBI) 215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Protocols

Product Specific Protocols
WB protocol for ABCD1 antibody 83038-4-RRDownload protocol
IF protocol for ABCD1 antibody 83038-4-RRDownload protocol
FC protocol for ABCD1 antibody 83038-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols