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IFNAR1 Recombinant antibody

IFNAR1 Uni-rAbTM Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230549C8

Cat no : 83002-4-RR

Synonyms

IFN alpha REC, Cytokine receptor class-II member 1, CRF2-1, CRF2 1, AVP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFNAR1 Recombinant antibody (83002-4-RR)

    WB analysis using 83002-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83002-4-RR (IFNAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using IFNAR1 Recombinant antibody (83002-4-RR)

    FC experiment of Jurkat using 83002-4-RR

    1x10^6 Jurkat cells were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of K562 using IFNAR1 Recombinant antibody (83002-4-RR)

    FC experiment of K562 using 83002-4-RR

    1x10^6 K562 were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 83002-4-RR

    Affinity and Kinetic Characterization of 83002-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83002-4-RR against Human IFNAR1 were performed. The affinity constant is 2.04 nM.

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HepG2 cells, HEK-293 cells, A431 cells, Jurkat cells
Positive FC detected inJurkat cells, K562

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83002-4-RR is IFNAR1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interferon (alpha, beta and omega) receptor 1
Calculated Molecular Weight 557 aa, 64 kDa
Observed Molecular Weight80-140 kDa
GenBank Accession NumberBC021825
Gene Symbol IFNAR1
Gene ID (NCBI) 3454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.

Protocols

Product Specific Protocols
WB protocol for IFNAR1 antibody 83002-4-RRDownload protocol
FC protocol for IFNAR1 antibody 83002-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols