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RILP Recombinant antibody

RILP Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230208G2

Cat no : 82996-4-RR

Synonyms

Rab-interacting lysosomal protein, PP10141, FLJ31193, 230208G2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RILP Recombinant antibody (82996-4-RR)

    WB analysis using 82996-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RILP Recombinant antibody (82996-4-RR)

    IHC staining of human ovarian cancer using 82996-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RILP Recombinant antibody (82996-4-RR)

    IHC staining of human ovarian cancer using 82996-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82996-4-RR

    Affinity and Kinetic Characterization of 82996-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82996-4-RR targets RILP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=1.04 x 10-11M
KOff=2.33 x 10-5M
KOn=2.05 x 106M
Immunogen RILP fusion protein Ag4472
Full Name Rab interacting lysosomal protein
Calculated Molecular Weight 401 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC031621
Gene Symbol RILP
Gene ID (NCBI) 83547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RILP antibody 82996-4-RRDownload protocol
IHC protocol for RILP antibody 82996-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols