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DDX60 Recombinant antibody

DDX60 Uni-rAbTM Recombinant Antibody for IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230293G10

Cat no : 82969-4-RR

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293G10

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DDX60 Recombinant antibody (82969-4-RR)

    IHC staining of human lung cancer using 82969-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DDX60 Recombinant antibody (82969-4-RR)

    IHC staining of human lung cancer using 82969-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using DDX60 Recombinant antibody (82969-4-RR)

    FC experiment of A431 using 82969-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human DDX60 (82969-4-RR, Clone:230293G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82969-4-RR targets DDX60 in IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX60 fusion protein Ag34406
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Calculated Molecular Weight 1712 aa, 198 kDa
Observed Molecular Weight 170-200 kDa
GenBank Accession NumberBC038115
Gene Symbol DDX60
Gene ID (NCBI) 55601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

Protocols

Product Specific Protocols
IHC protocol for DDX60 antibody 82969-4-RRDownload protocol
FC protocol for DDX60 antibody 82969-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols