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N-cadherin Recombinant antibody

N-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

230259H5

Cat no : 82968-1-RR

Synonyms

CDHN, CDH2, CD325, Cadherin-2, Cadherin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using N-cadherin Recombinant antibody (82968-1-RR)

    WB analysis using 82968-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using N-cadherin Recombinant antibody (82968-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using N-cadherin Recombinant antibody (82968-1-RR)

    IF Staining of rat brain using 82968-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using N-cadherin Recombinant antibody (82968-1-RR)

    IF Staining of HeLa using 82968-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82968-1-RR

    Affinity and Kinetic Characterization of 82968-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82968-1-RR against Human N-cadherin were performed. The affinity constant is 0.464 nM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82968-1-RR targets N-cadherin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated Molecular Weight100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001792.4
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

Protocols

Product Specific Protocols
WB protocol for N-cadherin antibody 82968-1-RRDownload protocol
IHC protocol for N-cadherin antibody 82968-1-RRDownload protocol
IF protocol for N-cadherin antibody 82968-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols