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ACAP1 Recombinant antibody

ACAP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230370D5

Cat no : 82967-4-RR

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1



Tested Applications

Positive WB detected inJurkat cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82967-4-RR targets ACAP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ACAP1 fusion protein Ag34309
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 82-85 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Protocols

Product Specific Protocols
WB protocol for ACAP1 antibody 82967-4-RRDownload protocol
IF protocol for ACAP1 antibody 82967-4-RRDownload protocol
FC protocol for ACAP1 antibody 82967-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols