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CLDN16 Recombinant antibody

CLDN16 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230193G3

Cat no : 82954-1-RR

Synonyms

PCLN-1, PCLN1, Paracellin-1, Claudin-16, 230193G3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLDN16 Recombinant antibody (82954-1-RR)

    IHC staining of mouse kidney using 82954-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLDN16 Recombinant antibody (82954-1-RR)

    IHC staining of mouse kidney using 82954-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CLDN16 Recombinant antibody (82954-1-RR)

    IF Staining of HEK-293 using 82954-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CLDN16 antibody (82954-1-RR, Clone: 230193G3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82954-1-RR targets CLDN16 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLDN16 fusion protein Ag26531
Full Name claudin 16
Calculated Molecular Weight 305 aa, 34 kDa
GenBank Accession NumberBC069682
Gene Symbol CLDN16
Gene ID (NCBI) 10686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CLDN16 antibody 82954-1-RRDownload protocol
IF protocol for CLDN16 antibody 82954-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols