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  • Featured Product
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HEBP1 Recombinant antibody

HEBP1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230156B6

Cat no : 82950-1-RR

Synonyms

Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP, HBP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEBP1 Recombinant antibody (82950-1-RR)

    WB analysis using 82950-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82950-1-RR (HEBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using HEBP1 Recombinant antibody (82950-1-RR)

    WB analysis of A549 using 82950-1-RR

    WB result of HEBP1 antibody (82950-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HEBP1 Recombinant antibody (82950-1-RR)

    IHC staining of mouse liver using 82950-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HEBP1 Recombinant antibody (82950-1-RR)

    IHC staining of mouse liver using 82950-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of U2OS cells using HEBP1 Recombinant antibody (82950-1-RR)

    FC experiment of U2OS using 82950-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug HEBP1 Recombinant antibody (82950-1-RR, Clone:230156B6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82950-1-RR

    Affinity and Kinetic Characterization of 82950-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82950-1-RR against Human HEBP1 were performed. The affinity constant is 74 nM.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, L02 cells, HepG2 cells, rat liver tissue. mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82950-1-RR targets HEBP1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEBP1 fusion protein Ag10538
Full Name heme binding protein 1
Calculated Molecular Weight 189 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC016277
Gene Symbol HEBP1
Gene ID (NCBI) 50865
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HEBP1 antibody 82950-1-RRDownload protocol
IHC protocol for HEBP1 antibody 82950-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols