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CAMP Recombinant antibody

CAMP Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230157C6

Cat no : 82940-2-RR

Synonyms

CAP18, Antibacterial peptide LL-23, Antibacterial peptide KS-30, Antibacterial peptide KR-20, Antibacterial peptide FF-33

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CAMP Recombinant antibody (82940-2-RR)

    FC experiment of MCF-7 using 82940-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Hela cells using CAMP Recombinant antibody (82940-2-RR)

    FC experiment of Hela using 82940-2-RR

    (blue)1x10^6 Hela cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution (red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82940-2-RR

    Affinity and Kinetic Characterization of 82940-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82940-2-RR against Human CAMP were performed. The affinity constant is 1.50 nM.

Tested Applications

Positive FC (Intra) detected inMCF-7 cells, Hela cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82940-2-RR targets CAMP in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CAMP fusion protein Ag2622
Full Name cathelicidin antimicrobial peptide
Calculated Molecular Weight 170 aa, 19 kDa
GenBank Accession NumberBC055089
Gene Symbol CAMP
Gene ID (NCBI) 820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

Protocols

Product Specific Protocols
FC protocol for CAMP antibody 82940-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols