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NFX1 Recombinant antibody

NFX1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230211H4

Cat no : 82924-2-RR

Synonyms

DKFZp779G2416, NFX1, NFX2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFX1 Recombinant antibody (82924-2-RR)

    WB analysis using 82924-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82924-2-RR (NFX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82924-2-RR

    Affinity and Kinetic Characterization of 82924-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82924-2-RR against Human NFX1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, K-562cells, HCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82924-2-RR targets NFX1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NFX1 fusion protein Ag9448
Full Name nuclear transcription factor, X-box binding 1
Calculated Molecular Weight 1120 aa, 124 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC012151
Gene Symbol NFX1
Gene ID (NCBI) 4799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NFX1 antibody 82924-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols