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HCRTR1 Recombinant antibody

HCRTR1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E18

Cat no : 82921-1-RR

Synonyms

Orexin receptor 1, Ox 1 R, Orexin/Hypocretin receptor type 1, Orexin receptor type 1, Hypocretin receptor type 1

Validation Data Gallery

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  • Western Blot (WB) analysis of C6 cells using HCRTR1 Recombinant antibody (82921-1-RR)

    WB analysis of C6 using 82921-1-RR

    C6 cells were subjected to SDS PAGE followed by western blot with 82921-1-RR (HCRTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82921-1-RR

    Affinity and Kinetic Characterization of 82921-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82921-1-RR against Human HCRTR1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82921-1-RR targets HCRTR1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HCRTR1 fusion protein Ag12269
Full Name hypocretin (orexin) receptor 1
Calculated Molecular Weight 425 aa, 48 kDa
Observed Molecular Weight54 kDa
GenBank Accession NumberBC074796
Gene Symbol HCRTR1
Gene ID (NCBI) 3061
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hypocretin receptor 1 (OX1R, also named HCRTR1) is a G protein-coupled receptor and is a critical participant in the regulation of motivated behavior (PMID:28971565). HCRTR1 is associated with the regulation of motivation, reward, and autonomic functions. HCRTR1 shows a selective binding affinity for HCRT1/orexin-A. Moreover, HCRTR1 and HCRTR2 share 64% sequence similarity in humans (PMID:34052813).

Protocols

Product Specific Protocols
WB protocol for HCRTR1 antibody 82921-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols