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ZC3H11A Recombinant antibody

ZC3H11A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230191H5

Cat no : 82912-1-RR

Synonyms

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191H5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZC3H11A Recombinant antibody (82912-1-RR)

    WB analysis using 82912-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82912-1-RR (ZC3H11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ZC3H11A Recombinant antibody (82912-1-RR)

    IHC staining of human ovary cancer using 82912-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82912-1-RR (ZC3H11A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZC3H11A Recombinant antibody (82912-1-RR)

    IF Staining of HepG2 using 82912-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZC3H11A antibody (82912-1-RR, Clone: 230191H5 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • Affinity and Kinetic Characterization of 82912-1-RR

    Affinity and Kinetic Characterization of 82912-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82912-1-RR against Human ZC3H11A were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, A549 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82912-1-RR targets ZC3H11A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZC3H11A fusion protein Ag23424
Full Name zinc finger CCCH-type containing 11A
Observed Molecular Weight100 kDa
GenBank Accession NumberBC014268
Gene Symbol ZC3H11A
Gene ID (NCBI) 9877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.

Protocols

Product Specific Protocols
WB protocol for ZC3H11A antibody 82912-1-RRDownload protocol
IHC protocol for ZC3H11A antibody 82912-1-RRDownload protocol
IF protocol for ZC3H11A antibody 82912-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols