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NSUN2 Recombinant antibody, PBS Only (Detector)

NSUN2 Recombinant Antibody for IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230186A6

Cat no : 82894-1-PBS

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN2 Recombinant antibody (82894-1-RR)

    IF Staining of HepG2 using 82894-1-RR (same clone as 82894-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-1-RR, Clone: 230186A6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00112-1

    Cytometric bead array standard curve of MP00112-1

    Cytometric bead array standard curve ofMP00112-1, NSUN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82894-4-PBS. Detection antibody: 82894-1-PBS. Standard: Ag14791. Range: 0.625-20 ng/mL.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82894-1-PBS targets NSUN2 as part of a matched antibody pair:

MP00112-1: 82894-4-PBS capture and 82894-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSUN2 fusion protein Ag14791
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated Molecular Weight 767 aa, 86 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.