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TRIM62 Recombinant antibody, PBS Only (Detector)

TRIM62 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230195G5

Cat no : 82892-1-PBS

Synonyms

Tripartite motif-containing protein 62, tripartite motif containing 62, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM62, 230195G5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TRIM62 Recombinant antibody (82892-1-RR)

    IF Staining of U2OS using 82892-1-RR (same clone as 82892-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM62 antibody (82892-1-RR, Clone: 230195G5 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82892-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TRIM62 Recombinant antibody (82892-1-RR)

    FC experiment of HeLa using 82892-1-RR (same clone as 82892-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TRIM62 (82892-1-RR, Clone:230195G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82892-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01312-1

    Cytometric bead array standard curve of MP01312-1

    Cytometric bead array standard curve of MP01312-1, TRIM62 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82892-5-PBS. Detection antibody: 82892-1-PBS. Standard: Ag7143. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 82892-1-RR

    Affinity and Kinetic Characterization of 82892-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82892-1-RR against Human TRIM62 were performed. The affinity constant is 90.8 pM.

Product Information

82892-1-PBS targets TRIM62 as part of a matched antibody pair:

MP01312-1: 82892-5-PBS capture and 82892-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM62 fusion protein Ag7143
Full Name tripartite motif-containing 62
Calculated Molecular Weight 54 kDa
GenBank Accession NumberBC001222
Gene Symbol TRIM62
Gene ID (NCBI) 55223
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).