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RBM17 Recombinant antibody

RBM17 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230125A10

Cat no : 82889-2-RR

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of MCF-7 cells using RBM17 Recombinant antibody (82889-2-RR)

    IP experiment of MCF-7 using 82889-2-RR

    IP result of anti-RBM17 (IP:82889-2-RR, 4ug; Detection:82889-2-RR 1:10000) with MCF-7 cells lysate 1470 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RBM17 Recombinant antibody (82889-2-RR)

    IHC staining of mouse testis using 82889-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RBM17 Recombinant antibody (82889-2-RR)

    IHC staining of mouse testis using 82889-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82889-2-RR (RBM17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM17 Recombinant antibody (82889-2-RR)

    IF Staining of HeLa using 82889-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM17 antibody (82889-2-RR, Clone: 230125A10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using RBM17 Recombinant antibody (82889-2-RR)

    FC experiment of A431 using 82889-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human RBM17 (82889-2-RR, Clone:230125A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82889-2-RR

    Affinity and Kinetic Characterization of 82889-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-2-RR against Human RBM17 were performed. The affinity constant is 2.86 nM.

Tested Applications

Positive IP detected inMCF-7 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82889-2-RR targets RBM17 in IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM17 fusion protein Ag0985
Full Name RNA binding motif protein 17
Calculated Molecular Weight 45 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

Protocols

Product Specific Protocols
IHC protocol for RBM17 antibody 82889-2-RRDownload protocol
IF protocol for RBM17 antibody 82889-2-RRDownload protocol
IP protocol for RBM17 antibody 82889-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols