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E2F7 Recombinant antibody

E2F7 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230073E3

Cat no : 82887-2-RR

Synonyms

Transcription factor E2F7, E2F-7, 230073E3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using E2F7 Recombinant antibody (82887-2-RR)

    WB analysis using 82887-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82887-2-RR (E2F7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using E2F7 Recombinant antibody (82887-2-RR)

    WB analysis of mouse kidney using 82887-2-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 82887-2-RR (E2F7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU2OS cells, mouse kidney tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82887-2-RR is E2F7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen E2F7 fusion protein Ag21599
Full Name E2F transcription factor 7
Calculated Molecular Weight 911 aa, 100 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC136366
Gene Symbol E2F7
Gene ID (NCBI) 144455
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E2F7, also named E2F transcription factor 7, is a 911 amino acid protein belonging to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells, and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 )

Protocols

Product Specific Protocols
WB protocol for E2F7 antibody 82887-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols