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MMP8 Recombinant antibody

MMP8 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230030G11

Cat no : 82885-1-RR

Synonyms

Matrix metalloproteinase-8, Matrix metalloproteinase 8, HNC, EC:3.4.24.34, CLG1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMP8 Recombinant antibody (82885-1-RR)

    IF Staining of HepG2 using 82885-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP8 antibody (82885-1-RR, Clone: 230030G11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82885-1-RR targets MMP8 in IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name matrix metallopeptidase 8 (neutrophil collagenase)
Calculated Molecular Weight 467 aa, 53 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC074988
Gene Symbol MMP8
Gene ID (NCBI) 4317
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa,respectively(PMID: 28772283, PMID:15042023).

Protocols

Product Specific Protocols
IF protocol for MMP8 antibody 82885-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols