Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL6 antibody (82877-2-RR, Clone: 230162E11 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL6 antibody (82877-2-RR, Clone: 230162E11 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of U2OS using 82877-2-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug APOL6 Recombinant antibody (82877-2-RR, Clone:230162E11)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 U2OS cells were intracellularly stained with 0.25 ug APOL6 Recombinant antibody (82877-2-RR, Clone:230162E11)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 82877-2-RR
Biolayer interferometry (BLl) kinetic assays of 82877-2-RR against Human APOL6 were performed. The affinity constant is 13.8 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using APOL6 antibody (82877-2-RR, Clone: 230162E11 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC (INTRA) Figures
FC experiment of U2OS using 82877-2-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug APOL6 Recombinant antibody (82877-2-RR, Clone:230162E11)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 82877-2-RR
Biolayer interferometry (BLl) kinetic assays of 82877-2-RR against Human APOL6 were performed. The affinity constant is 13.8 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
APOL6 Recombinant antibody
Catalog Number
82877-2-RR
Citations
-
Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
