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ZO1 Recombinant antibody, PBS Only (Detector)

ZO1 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240150C5

Cat no : 82870-9-PBS

Synonyms

TJP1, ZO-1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00349-4

    Sandwich ELISA standard curve of MP00349-4

    Sandwich ELISA standard curve of MP00349-4, Human ZO-1 Recombinant Matched Antibody Pair - PBS only. 82870-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33182. 82870-9-PBS was HRP conjugated as the detection antibody. Range: 125-8000 pg/mL.

  • Sample test of MP00349-4

    Sample test of MP00349-4

    Serum of nine individual healthy human donors was measured. The ZO-1 concentration of detected samples was determined to be 352.4 pg/mL with a range of 83.4 - 1,272.8 pg/mL.

  • Sample test of MP00349-4

    Sample test of MP00349-4

    The mean ZO-1 concentration was determined to be 7.5 ng/mL in bEnd.3 cell extract based on a 1.8 mg/mL extract load.

Product Information

82870-9-PBS targets ZO1 as part of a matched antibody pair:

MP00349-4: 82870-2-PBS capture and 82870-9-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZO1 fusion protein Ag33182
Full Name tight junction protein 1 (zona occludens 1)
Calculated Molecular Weight 1748 aa, 195 kDa
GenBank Accession NumberBC111712
Gene Symbol ZO-1
Gene ID (NCBI) 7082
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.