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  • Western Blot (WB) analysis of various lysates using BACH1 Recombinant antibody (82842-7-RR)

    WB analysis using 82842-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BACH1 Recombinant antibody (82842-7-RR)

    IF Staining of HeLa using 82842-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

BACH1 Recombinant antibody

BACH1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82842-7-RR
Clone No.4K14

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

BACH-1, BACH 1, 4K14

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, Raji cells, rat brain tissue, pig brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82842-7-RR targets BACH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BACH1 fusion protein Ag5128 Predict reactive species
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated Molecular Weight 82 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC063307
Gene Symbol BACH1
Gene ID (NCBI) 571
ENSEMBL Gene IDENSG00000156273
RRIDAB_3086551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14867
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Protocols

Product Specific Protocols
WB protocol for BACH1 antibody 82842-7-RRDownload protocol
IF protocol for BACH1 antibody 82842-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols