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Mono-Methyl-Histone H3 (Lys36) Recombinant antibody

Mono-Methyl-Histone H3 (Lys36) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241138B6

Cat no : 82825-2-RR

Synonyms

HIST1H3A, 241138B6, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    WB analysis using 82825-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82825-2-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using Mono-Methyl-Histone H3 (Lys36) Recombinant antibod (82825-2-RR)

    FC experiment of HeLa using 82825-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (82825-2-RR, Clone:241138B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, THP-1 cells, K-562 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82825-2-RR targets Mono-Methyl-Histone H3 (Lys36) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name histone cluster 1, H3a
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

Protocols

Product Specific Protocols
WB protocol for Mono-Methyl-Histone H3 (Lys36) antibody 82825-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols