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GH1 Recombinant antibody

GH1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4G8

Cat no : 82813-2-RR

Synonyms

GH, 4G8, growth hormone 1, Growth hormone, GH-N

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GH1 Recombinant antibody (82813-2-RR)

    WB analysis using 82813-2-RR

    Human placenta tissue was subjected to SDS PAGE followed by western blot with 82813-2-RR (GH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GH1 Recombinant antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GH1 Recombinant antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82813-2-RR

    Affinity and Kinetic Characterization of 82813-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82813-2-RR against Human GH1 were performed. The affinity constant is 0.76 nM.

Tested Applications

Positive WB detected inhuman saliva
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82813-2-RR targets GH1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name GH1
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC075012
Gene Symbol GH1
Gene ID (NCBI) 2688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Protocols

Product Specific Protocols
WB protocol for GH1 antibody 82813-2-RRDownload protocol
IHC protocol for GH1 antibody 82813-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols