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OLIG2 Recombinant antibody

OLIG2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240839C1

Cat no : 82806-7-RR

Synonyms

Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1, 5O7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OLIG2 Recombinant antibody (82806-7-RR)

    WB analysis using 82806-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82806-7-RR (OLIG2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82806-7-RR

    Affinity and Kinetic Characterization of 82806-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82806-7-RR against Human OLIG2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inTHP-1 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82806-7-RR is OLIG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OLIG2 fusion protein Ag5089
Full Name oligodendrocyte lineage transcription factor 2
Calculated Molecular Weight 32 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC047511
Gene Symbol OLIG2
Gene ID (NCBI) 10215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

Protocols

Product Specific Protocols
WB protocol for OLIG2 antibody 82806-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols