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ZDHHC9 Recombinant antibody

ZDHHC9 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

9G5

Cat no : 82739-1-RR

Synonyms

DHHC-9, DHHC9, DHHC 9, CXorf11, CGI-89

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZDHHC9 Recombinant antibody (82739-1-RR)

    WB analysis using 82739-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82739-1-RR (ZDHHC9 antibody) at dilution of 1:2700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZDHHC9 Recombinant antibody (82739-1-RR)

    WB analysis using 82739-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82739-1-RR (ZDHHC9 antibody) at dilution of 1:2700 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82739-1-RR

    Affinity and Kinetic Characterization of 82739-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82739-1-RR against Human ZDHHC9 were performed. The affinity constant is 0.344 nM.

Tested Applications

Positive WB detected inmouse brain tissue, SK-N-SH cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82739-1-RR targets ZDHHC9 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZDHHC9 fusion protein Ag20881
Full Name zinc finger, DHHC-type containing 9
Calculated Molecular Weight 41 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC006200
Gene Symbol ZDHHC9
Gene ID (NCBI) 51114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

Protocols

Product Specific Protocols
WB protocol for ZDHHC9 antibody 82739-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols