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ATG13 Recombinant antibody

ATG13 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G14

Cat no : 82718-3-RR

Synonyms

KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 3G14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG13 Recombinant antibody (82718-3-RR)

    WB analysis using 82718-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82718-3-RR (KIAA0652 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ATG13 Recombinant antibody (82718-3-RR)

    WB analysis of HEK-293 using 82718-3-RR

    WB result of ATG13 antibody (82718-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82718-3-RR

    Affinity and Kinetic Characterization of 82718-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82718-3-RR against Human ATG13 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HEK- 293 cells, BGC-823 cells, SH-SY5Y cells, Jurkat cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82718-3-RR targets ATG13 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATG13 fusion protein Ag13090
Full Name KIAA0652
Calculated Molecular Weight 57 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC001331
Gene Symbol ATG13
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Protocols

Product Specific Protocols
WB protocol for ATG13 antibody 82718-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols