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EIF6 Recombinant antibody

EIF6 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G23

Cat no : 82693-1-RR

Synonyms

integrin beta 4 binding protein, CAB, B4 integrin interactor, B(2)GCN homolog, b(2)gcn

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF6 Recombinant antibody (82693-1-RR)

    WB analysis using 82693-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82693-1-RR (EIF6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82693-1-RR

    Affinity and Kinetic Characterization of 82693-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82693-1-RR against Human EIF6 were performed. The affinity constant is 6.29 nM.

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, A375 cells, A549 cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82693-1-RR targets EIF6 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF6 fusion protein Ag0324
Full Name eukaryotic translation initiation factor 6
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC001119
Gene Symbol EIF6
Gene ID (NCBI) 3692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), it functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.

Protocols

Product Specific Protocols
WB protocol for EIF6 antibody 82693-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols