• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RPL14 Recombinant antibody

RPL14 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2K22

Cat no : 82688-1-RR

Synonyms

L14, hRL14, CTG B33, CAG-ISL 7, CAG ISL 7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RPL14 Recombinant antibody (82688-1-RR)

    WB analysis using 82688-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82688-1-RR (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL14 Recombinant antibody (82688-1-RR)

    IF Staining of HepG2 using 82688-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL14 antibody (82688-1-RR, Clone: 2K22 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82688-1-RR

    Affinity and Kinetic Characterization of 82688-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82688-1-RR against Human RPL14 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, A431 cells, MDA-MB-453s cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82688-1-RR targets RPL14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL14 fusion protein Ag6976
Full Name ribosomal protein L14
Calculated Molecular Weight 23 kDa
Observed Molecular Weight23-27 kDa
GenBank Accession NumberBC000606
Gene Symbol RPL14
Gene ID (NCBI) 9045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

Protocols

Product Specific Protocols
WB protocol for RPL14 antibody 82688-1-RRDownload protocol
IF protocol for RPL14 antibody 82688-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols