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  • Western Blot (WB) analysis of various lysates using TGM2 Recombinant antibody (82552-4-RR)

    WB analysis using 82552-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82552-4-RR (TGM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TGM2 Recombinant antibody (82552-4-RR)

    IHC staining of human stomach cancer using 82552-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82552-4-RR (TGM2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82552-4-RR

    Affinity and Kinetic Characterization of 82552-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82552-4-RR against Human TGM2 were performed. The affinity constant is below 1 pM.

TGM2 Recombinant antibody

TGM2 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA
Cat No. 82552-4-RR
Clone No.242828D3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

242828D3, Erythrocyte transglutaminase, TG(C), TG2, TGase 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, U2OS cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82552-4-RR targets TGM2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TGM2 fusion protein Ag7439 Predict reactive species
Full Name transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Calculated Molecular Weight 77 kDa
Observed Molecular Weight 77-80 kDa
GenBank Accession NumberBC003551
Gene Symbol TGM2
Gene ID (NCBI) 7052
ENSEMBL Gene IDENSG00000198959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21980
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TGM2 antibody 82552-4-RRDownload protocol
IHC protocol for TGM2 antibody 82552-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols