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EIF3D Recombinant antibody

EIF3D Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3O8

Cat no : 82512-1-RR

Synonyms

eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF3D Recombinant antibody (82512-1-RR)

    WB analysis using 82512-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using EIF3D Recombinant antibody (82512-1-RR)

    WB analysis of rat liver using 82512-1-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:30300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using EIF3D Recombinant antibody (82512-1-RR)

    IP experiment of A549 using 82512-1-RR

    IP result of anti-EIF3D (IP:82512-1-RR, 4ug; Detection:82512-1-RR 1:5000) with A549 cells lysate 920 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3D Recombinant antibody (82512-1-RR)

    IHC staining of human breast cancer using 82512-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3D Recombinant antibody (82512-1-RR)

    IHC staining of human breast cancer using 82512-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EIF3D Recombinant antibody (82512-1-RR)

    IF Staining of U2OS using 82512-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EIF3D Recombinant antibody (82512-1-RR)

    IF Staining of U2OS using 82512-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82512-1-RR

    Affinity and Kinetic Characterization of 82512-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82512-1-RR against Human EIF3D were performed. The affinity constant is 5.83 nM.

Tested Applications

Positive WB detected inHeLa cells, rat liver tissue, HepG2 cells, A549 cells, A431 cells, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82512-1-RR targets EIF3D in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EIF3D fusion protein Ag0268
Full Name eukaryotic translation initiation factor 3, subunit D
Calculated Molecular Weight 66 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC000328
Gene Symbol EIF3D
Gene ID (NCBI) 8664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

Protocols

Product Specific Protocols
WB protocol for EIF3D antibody 82512-1-RRDownload protocol
IHC protocol for EIF3D antibody 82512-1-RRDownload protocol
IF protocol for EIF3D antibody 82512-1-RRDownload protocol
IP protocol for EIF3D antibody 82512-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols