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Aquaporin 4 Recombinant antibody

Aquaporin 4 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240856C7

Cat no : 82296-6-RR

Synonyms

AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Aquaporin 4 Recombinant antibody (82296-6-RR)

    WB analysis using 82296-6-RR

    37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Aquaporin 4 Recombinant antibody (82296-6-RR)

    IHC staining of rat brain using 82296-6-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Aquaporin 4 Recombinant antibody (82296-6-RR)

    IHC staining of rat brain using 82296-6-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82296-6-RR

    Affinity and Kinetic Characterization of 82296-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82296-6-RR against Human Aquaporin 4 were performed. The affinity constant is 5.06 nM.

Tested Applications

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82296-6-RR is Aquaporin 4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Aquaporin 4 fusion protein Ag9561
Full Name aquaporin 4
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight 32-34 kDa
GenBank Accession NumberBC022286
Gene Symbol AQP4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for Aquaporin 4 antibody 82296-6-RRDownload protocol
IHC protocol for Aquaporin 4 antibody 82296-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols