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SEPT4 Recombinant antibody

SEPT4 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5O8

Cat no : 82284-1-RR

Synonyms

hCDCREL-2, hCDCREL2, Cell division control-related protein 2, C17orf47, BRADEION

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT4 Recombinant antibody (82284-1-RR)

    WB analysis using 82284-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82284-1-RR (SEPT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEPT4 Recombinant antibody (82284-1-RR)

    IHC staining of mouse brain using 82284-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEPT4 Recombinant antibody (82284-1-RR)

    IHC staining of mouse brain using 82284-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82284-1-RR (SEPT4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82284-1-RR

    Affinity and Kinetic Characterization of 82284-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82284-1-RR against Human SEPT4 were performed. The affinity constant is 1.38 nM.

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82284-1-RR targets SEPT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEPT4 fusion protein Ag3185
Full Name septin 4
Calculated Molecular Weight 459 aa, 53 kDa
Observed Molecular Weight38-55 kDa
GenBank Accession NumberBC018056
Gene Symbol SEPT4
Gene ID (NCBI) 5414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).

Protocols

Product Specific Protocols
WB protocol for SEPT4 antibody 82284-1-RRDownload protocol
IHC protocol for SEPT4 antibody 82284-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols