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HNRNPL Recombinant antibody

HNRNPL Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3J22

Cat no : 82259-1-RR

Synonyms

P/OKcl.14, HNRPL, hnRNP L, 3J22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPL Recombinant antibody (82259-1-RR)

    WB analysis using 82259-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82259-1-RR (HNRNPL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HNRNPL Recombinant antibody (82259-1-RR)

    IHC staining of human stomach cancer using 82259-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HNRNPL Recombinant antibody (82259-1-RR)

    IHC staining of human stomach cancer using 82259-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HNRNPL Recombinant antibody (82259-1-RR)

    IHC staining of human kidney using 82259-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HNRNPL Recombinant antibody (82259-1-RR)

    IHC staining of human kidney using 82259-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPL Recombinant antibody (82259-1-RR)

    IHC staining of human colon cancer using 82259-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPL Recombinant antibody (82259-1-RR)

    IHC staining of human colon cancer using 82259-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82259-1-RR (HNRNPL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82259-1-RR

    Affinity and Kinetic Characterization of 82259-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82259-1-RR against Human HNRNPL were performed. The affinity constant is 6.54 nM.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, PC-3 cells, BxPC-3 cells, C6 cells, HSC-T6 cells
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:19600
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82259-1-RR targets HNRNPL in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRNPL fusion protein Ag13211
Full Name heterogeneous nuclear ribonucleoprotein L
Calculated Molecular Weight 572 aa, 62 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC069184
Gene Symbol HNRNPL
Gene ID (NCBI) 3191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. It is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. The two isoforms of hnRNP L are generated by alternative splicing. The human hnRNPL gene encodes a protein that is localized in the nucleoplasm and cytoplasm. This antibody may recognize both isoforms of the hnRNP L protein.

Protocols

Product Specific Protocols
WB protocol for HNRNPL antibody 82259-1-RRDownload protocol
IHC protocol for HNRNPL antibody 82259-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols