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Mouse Il10 Recombinant antibody, PBS Only (Capture)

Il10 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240430A11

Cat no : 82191-5-PBS

Synonyms

IL-10,IL10, IL-10, interleukin 10, Il 10, CSIF

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00507-4

    Sandwich ELISA standard curve of MP00507-4

    Sandwich ELISA standard curve of MP00507-4, IL-10 Recombinant Matched Antibody Pair - PBS only. 82191-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0026.82191-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00507-4

    Sample test of MP00507-4

    Mouse splenocytes (1 x 10^cells/mL) were cultured for 4 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS. The mean IL-10 concentration was determined to be 7.59 pg/mL in unstimulated spleen cell culture supernatant, 2,461.0 pg/mL in IFN-γ and LPS stimulated spleen cell culture supernatant. Mouse splenocytes (1 x 10^cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum,1 μg/mL of LPS. The mean IL-10 concentration was determined to be 11.30 pg/mL in unstimulated spleen cell culture supernatant, 143.99 pg/mL in LPS stimulated spleen cell culture supernatant.

  • Cytometric bead array standard curve of MP00507-1

    Cytometric bead array standard curve of MP00507-1

    Cytometric bead array standard curve of MP00507-1, MOUSE IL-10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82191-5-PBS. Detection antibody: 82191-2-PBS. Standard: Eg0026. Range: 0.625-80 ng/mL.

Product Information

82191-5-PBS targets Il10 as part of a matched antibody pair:

MP00507-1: 82191-5-PBS capture and 82191-2-PBS detection (validated in Cytometric bead array)

MP00507-4: 82191-5-PBS capture and 82191-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 10
Calculated Molecular Weight 21 kDa
GenBank Accession NumberNM_010548
Gene Symbol Il10
Gene ID (NCBI) 16153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.