DDX3 Recombinant antibody
DDX3 Recombinant Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5C12
Cat no : 81903-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells, mouse brain tissue, rat brain tissue |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
81903-1-RR targets DDX3 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | DDX3 fusion protein Ag1614 |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked |
Calculated Molecular Weight | 73 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC011819 |
Gene Symbol | DDX3 |
Gene ID (NCBI) | 1654 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.
Protocols
Product Specific Protocols | |
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WB protocol for DDX3 antibody 81903-1-RR | Download protocol |
IF protocol for DDX3 antibody 81903-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |