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Phospho-4EBP1 (Thr37) Recombinant antibody, PBS Only (Capture)

Phospho-4EBP1 (Thr37) Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240535G3

Cat no : 81812-4-PBS

Synonyms

EIF4EBP1, eIF4E binding protein 1, BP 1, 4E-BP1, 4EBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-4EBP1 (Thr37) Recombinant antibody (81812-4-RR)

    WB analysis using 81812-4-RR (same clone as 81812-4-PBS)

    Non-treated HEK-293T cells, phosphatase inhibitor treated HEK-293T cells and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81812-4-RR (Phospho-4EBP1 (Thr37) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81812-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00559-2

    Cytometric bead array standard curve of MP00559-2

    Cytometric bead array standard curve of MP00559-2, Phospho-4EBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81812-4-PBS. Detection antibody: 81812-3-PBS. Standard: SY01767P. Range: 0.78-50 ng/mL.

Product Information

81812-4-PBS targets Phospho-4EBP1 (Thr37) as part of a matched antibody pair:

MP00559-2: 81812-4-PBS capture and 81812-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 4E binding protein 1
Calculated Molecular Weight 118 aa, 12 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC004459
Gene Symbol EIF4EBP1
Gene ID (NCBI) 1978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)